Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations

Toward Quantitative “In Vivo Biochemistry” with Fluorescence Fluctuation Spectroscopy

Quantitative description of protein dynamics and interactions in vivo with temporal and spatial resolution is a key step in dissecting molecular mechanisms in cell biology. Fluorescence fluctuation spectroscopy (FFS) has recently emerged as a powerful in vivo tool for assessing molecular concentration and movement and formation of hetero- and homo-oligomeric complexes. This article discusses po...

From Micro to Nanorheology: Basic Principles of Microrheology Measurements with Fluorescence Correlation Spectroscopy

Fluorescence correlation spectroscopy (FCS) represents one of the most sensitive fluorescence techniques due to single molecule detection. Microrheology serves as a tool to study viscoelastic properties of microenvironment of observed particles. We used 100 nm latex particles to study mean square displacement (MSD) of these particles in Newtonian fluids. The comparison of MSD determined by FCS ...

Teaching Basic Principles of Neuroscience with Computer Simulations

It is generally believed that students learn best through activities that require their direct participation. By using simulations as a tool for learning neuroscience, students are directly engaged in the activity and obtain immediate feedback and reinforcement. This paper describes a series of biophysical models and computer simulations that can be used by educators and students to explore a v...

Principles of Fluorescence Spectroscopy Third Edition

Teaching Robots through Situated Interactive Dialogue and Visual Demonstrations

The ability to quickly adapt to new environments and incorporate new knowledge is of great importance for robots operating in unstructured environments and interacting with non-expert users. This paper reports on our current progress in tackling this problem. We propose the development of a framework for teaching robots to perform tasks using natural language instructions, visual demonstrations...